In a comparative study, Klein and colleagues (1994) studied the overall fat metabolism ratios for endurance-trained and untrained men during low-intensity exercise routines. They sample a total of 10 men, 5 who were endurance-trained athletes and 5 who were untrained. They started off by calculating and analyzing the overall lipid activities and muscle activities while all 10 subjects were resting before the exercise routines began. They also calculated the free fatty acid (FFA) and glycerol rate of appearance (Ra) ratios. The calculated the lipid activities using indirect calorimetry as a determinant and the FFA and glycerol levels through the combination of glycerol (2H5) and palpitate (1-13C) one after another (Klein et al., 1994).

The results showed that the lipolytic reaction after 4 hours of high-intensity training routines showed no significant variations in the overall FFA and glycerol levels between the endurance-trained subjects (increase in a minimum of 1.02/kg to 3.76/kg...
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