" (Volpicelli-Daley and Levey, 2003)

Prior to visualization of the molecule of interest it is necessary to "fix and section the brain tissue. Double-labeling immunofluorescence is stated to detect "localization of a protein of interest as well as the distribution of the protein relative to another marker such as a neurochemical or organelle marker." (Volpicelli-Daley and Levey, 2003 Fluorescence imaging labeled tissue through use of confocal makes provision of "high-resolution analysis of the extent of colocalization, with a theoretical limit of resolution of 0.1 to 0.2 um." (Volpicelli-Daley and Levey, 2003) Immunofluorescence techniques are stated to "in general...utilize secondary antibodies conjugated to a flurosphore." (Volpicelli-Daley and Levey, 2003) It is important according to Volpicelli-Daley and Levey to choose flurosphores with "minimal background staining and a minimum overlap of excitation/emission spectra...when performing double labeling experiments." (2003)

IV. FLUORESCENCE MICROSCOPY

The work of Coling and Kachar (1997) entitled: "Theory and Application of...
[ View Full Essay]