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Following these general examinations and the more extensive examinations of cardiovascular health, specifically identified areas in participants' mouths will be variously swabbed or scraped to obtain bacteria samples, and levels of bacteria in these areas and in the mouth as a whole will be measured using a variety of techniques. If necessary, the bacteria collected through this direct methodology will be cultured in an appropriate lab setting using standard equipment, with twenty-four hours being sufficient time for the culture to grow to be identifiable in most instances. Examination of bacteria types, growth patterns in the mouth, and correlation with cardiovascular disease will be identifiable with the data collected via these methods, enabling appropriate analysis and a determination of correlation.
The latest available version of SPSS software operated by an experienced statistician, under the observation and supervision of the lead researcher, will be utilized to analyze the raw data collected in this study. Specifically, regression analyses will be utilized in order to determine the correlation, if any, that exists between the existence of certain types or levels of bacteria in the mouth or in certain areas of the mouth and cardiovascular disease. The Mann-Whitney test will also be used to determine which if any variables are statistically different between the participant group with cardiovascular disease and those without. Again, this is in keeping with similarly designed studies already successfully completed (Johansson et al. 2008).